Device

Part:BBa_K1819007

Designed by: Laís Ribovski   Group: iGEM15_Brasil-USP   (2015-09-16)


Constitutive promoter (J23117) and GFP (S05308)

Constitutive promotor from Anderson library (BBa_J23117) assembled to green fluorescent protein attached to linker (BBa_S05308). This part was used to characterize BBa_K1819000 and compare its efficiency to green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (BBa_E0040).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 717


[edit]
Categories
//chassis/prokaryote/ecoli
//function/reporter
//function/reporter/fluorescence
Parameters
colorGreen
directionForward
emission
emit511
excitation
excite501
kegg
lum
proteinGFPmut3b
swisspro